diethylpyrocarbonate depc treated rnase free water (Qiagen)
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Diethylpyrocarbonate Depc Treated Rnase Free Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnase+free+water/RNase-Free+Water/pm42860416-493-24-28
Average 99 stars, based on 5527 article reviews
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Gene Expression:Article Title: Natural Killer Cell-Derived Extracellular Vesicles Exhibit Cytotoxicity Against Bulk Tumor Cells and Cancer Stem Cells in Triple-Negative Breast Cancer Article Snippet: Total RNA was extracted using the RNeasy Mini kit (Qiagen, Toronto, ON, Canada). cDNA was obtained from RNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) as per the manufacturer’s instructions. .. Gene expression was measured using the CFX Opus 96 Real-Time PCR System (V5.3.022.1030; Bio-Rad, Hercules, CA, USA) in a reaction mixture consisting of 50% SyBr Green Supermix (Bio-Rad, Hercules, CA, USA), 37.5% Real-time Polymerase Chain Reaction:Article Title: Natural Killer Cell-Derived Extracellular Vesicles Exhibit Cytotoxicity Against Bulk Tumor Cells and Cancer Stem Cells in Triple-Negative Breast Cancer Article Snippet: Total RNA was extracted using the RNeasy Mini kit (Qiagen, Toronto, ON, Canada). cDNA was obtained from RNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) as per the manufacturer’s instructions. .. Gene expression was measured using the CFX Opus 96 Real-Time PCR System (V5.3.022.1030; Bio-Rad, Hercules, CA, USA) in a reaction mixture consisting of 50% SyBr Green Supermix (Bio-Rad, Hercules, CA, USA), 37.5% Article Title: Night shift work and epigenetic modifications: cardiovascular-related miRNA expression within a European cohort of night shift workers. Article Snippet: .. The qPCR amplification mixtures contained 3 μL diluted template cDNA, 5 μL 2x miRCURY SYBR Green master mix, 0.5 μL ROX reference dye, 0.5 μL SYBR Green Assay:Article Title: Natural Killer Cell-Derived Extracellular Vesicles Exhibit Cytotoxicity Against Bulk Tumor Cells and Cancer Stem Cells in Triple-Negative Breast Cancer Article Snippet: Total RNA was extracted using the RNeasy Mini kit (Qiagen, Toronto, ON, Canada). cDNA was obtained from RNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) as per the manufacturer’s instructions. .. Gene expression was measured using the CFX Opus 96 Real-Time PCR System (V5.3.022.1030; Bio-Rad, Hercules, CA, USA) in a reaction mixture consisting of 50% SyBr Green Supermix (Bio-Rad, Hercules, CA, USA), 37.5% Article Title: Night shift work and epigenetic modifications: cardiovascular-related miRNA expression within a European cohort of night shift workers. Article Snippet: .. The qPCR amplification mixtures contained 3 μL diluted template cDNA, 5 μL 2x miRCURY SYBR Green master mix, 0.5 μL ROX reference dye, 0.5 μL Polymerase Chain Reaction:Article Title: Diagnostic value of digital PCR (dPCR) in the diagnosis of leishmania infantum from canine chronic dermatitis. Article Snippet: A dPCR Leishmania TaqMan-assay was set up with the same primer pair and probe as described in Vitale et al. (2004) using the QIAcuity Probe PCR kit (Qiagen, Hilden, Germany) in a QIAcuity One dPCR thermal cycler (Qiagen, Hilden, Germany). dPCR reactions were performed using 26 k 8-well nanoplates, with a final reaction volume of 40 μL per well. .. Each reaction mix contained: 10 μL of 4× Probe PCR Master Mix (Qiagen, Hilden, Germany); 4 μL of a 10× primer-probe mix comprising 0.8 μM forward primer, 0.8 μM reverse primer, and 0.4 μM fluorogenic probe (Eurofins Genomics, Ebersberg, Germany); 10 ng of template DNA; and Article Title: Hemoglobinuria associated with Candidatus Mycoplasma Haemobos in a primiparous Holstein cow in Turkey: a case report Article Snippet: .. PCR amplification was performed in a 25 μL containing DNase- and Article Title: Night shift work and epigenetic modifications: cardiovascular-related miRNA expression within a European cohort of night shift workers. Article Snippet: .. The qPCR amplification mixtures contained 3 μL diluted template cDNA, 5 μL 2x miRCURY SYBR Green master mix, 0.5 μL ROX reference dye, 0.5 μL Amplification:Article Title: Hemoglobinuria associated with Candidatus Mycoplasma Haemobos in a primiparous Holstein cow in Turkey: a case report Article Snippet: .. PCR amplification was performed in a 25 μL containing DNase- and Article Title: Night shift work and epigenetic modifications: cardiovascular-related miRNA expression within a European cohort of night shift workers. Article Snippet: .. The qPCR amplification mixtures contained 3 μL diluted template cDNA, 5 μL 2x miRCURY SYBR Green master mix, 0.5 μL ROX reference dye, 0.5 μL |
