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diethylpyrocarbonate depc treated rnase free water  (Qiagen)


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    Structured Review

    Qiagen diethylpyrocarbonate depc treated rnase free water
    Diethylpyrocarbonate Depc Treated Rnase Free Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase+free+water/RNase-Free+Water/pm42860416-493-24-28
    Average 99 stars, based on 5527 article reviews
    diethylpyrocarbonate depc treated rnase free water - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Natural Killer Cell-Derived Extracellular Vesicles Exhibit Cytotoxicity Against Bulk Tumor Cells and Cancer Stem Cells in Triple-Negative Breast Cancer
    Article Snippet: Total RNA was extracted using the RNeasy Mini kit (Qiagen, Toronto, ON, Canada). cDNA was obtained from RNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) as per the manufacturer’s instructions. .. Gene expression was measured using the CFX Opus 96 Real-Time PCR System (V5.3.022.1030; Bio-Rad, Hercules, CA, USA) in a reaction mixture consisting of 50% SyBr Green Supermix (Bio-Rad, Hercules, CA, USA), 37.5% RNAse-free water (Qiagen, Toronto, ON, Canada), 5% forward and reverse primers (all primers were purchased from Eurofin Genomics, Louisville, KY, USA), and 2.5% cDNA. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Natural Killer Cell-Derived Extracellular Vesicles Exhibit Cytotoxicity Against Bulk Tumor Cells and Cancer Stem Cells in Triple-Negative Breast Cancer
    Article Snippet: Total RNA was extracted using the RNeasy Mini kit (Qiagen, Toronto, ON, Canada). cDNA was obtained from RNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) as per the manufacturer’s instructions. .. Gene expression was measured using the CFX Opus 96 Real-Time PCR System (V5.3.022.1030; Bio-Rad, Hercules, CA, USA) in a reaction mixture consisting of 50% SyBr Green Supermix (Bio-Rad, Hercules, CA, USA), 37.5% RNAse-free water (Qiagen, Toronto, ON, Canada), 5% forward and reverse primers (all primers were purchased from Eurofin Genomics, Louisville, KY, USA), and 2.5% cDNA. ..

    Article Title: Night shift work and epigenetic modifications: cardiovascular-related miRNA expression within a European cohort of night shift workers.
    Article Snippet: .. The qPCR amplification mixtures contained 3 μL diluted template cDNA, 5 μL 2x miRCURY SYBR Green master mix, 0.5 μL ROX reference dye, 0.5 μL RNAse free water and 1 μL miRCURY LNA miRNA PCR Assay (Qiagen). ..

    SYBR Green Assay:

    Article Title: Natural Killer Cell-Derived Extracellular Vesicles Exhibit Cytotoxicity Against Bulk Tumor Cells and Cancer Stem Cells in Triple-Negative Breast Cancer
    Article Snippet: Total RNA was extracted using the RNeasy Mini kit (Qiagen, Toronto, ON, Canada). cDNA was obtained from RNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) as per the manufacturer’s instructions. .. Gene expression was measured using the CFX Opus 96 Real-Time PCR System (V5.3.022.1030; Bio-Rad, Hercules, CA, USA) in a reaction mixture consisting of 50% SyBr Green Supermix (Bio-Rad, Hercules, CA, USA), 37.5% RNAse-free water (Qiagen, Toronto, ON, Canada), 5% forward and reverse primers (all primers were purchased from Eurofin Genomics, Louisville, KY, USA), and 2.5% cDNA. ..

    Article Title: Night shift work and epigenetic modifications: cardiovascular-related miRNA expression within a European cohort of night shift workers.
    Article Snippet: .. The qPCR amplification mixtures contained 3 μL diluted template cDNA, 5 μL 2x miRCURY SYBR Green master mix, 0.5 μL ROX reference dye, 0.5 μL RNAse free water and 1 μL miRCURY LNA miRNA PCR Assay (Qiagen). ..

    Polymerase Chain Reaction:

    Article Title: Diagnostic value of digital PCR (dPCR) in the diagnosis of leishmania infantum from canine chronic dermatitis.
    Article Snippet: A dPCR Leishmania TaqMan-assay was set up with the same primer pair and probe as described in Vitale et al. (2004) using the QIAcuity Probe PCR kit (Qiagen, Hilden, Germany) in a QIAcuity One dPCR thermal cycler (Qiagen, Hilden, Germany). dPCR reactions were performed using 26 k 8-well nanoplates, with a final reaction volume of 40 μL per well. .. Each reaction mix contained: 10 μL of 4× Probe PCR Master Mix (Qiagen, Hilden, Germany); 4 μL of a 10× primer-probe mix comprising 0.8 μM forward primer, 0.8 μM reverse primer, and 0.4 μM fluorogenic probe (Eurofins Genomics, Ebersberg, Germany); 10 ng of template DNA; and RNase-free water (Qiagen, Hilden, Germany) to the final volume of 40 μL. ..

    Article Title: Hemoglobinuria associated with Candidatus Mycoplasma Haemobos in a primiparous Holstein cow in Turkey: a case report
    Article Snippet: .. PCR amplification was performed in a 25 μL containing DNase- and RNase-free water (Qiagen ® , Germany), 10× PCR buffer (Thermo ScientificTM, Lithuania), MgCl2 (25 mM; Thermo ScientificTM, Lithuania), 200 μM of each deoxynucleotide triphosphate (dNTP; Thermo ScientificTM, Lithuania), 1.25 U Taq DNA polymerase (Thermo ScientificTM, Lithuania), 1 μL of each primer (10 pmol/μL), and 3 μL of template DNA. ..

    Article Title: Night shift work and epigenetic modifications: cardiovascular-related miRNA expression within a European cohort of night shift workers.
    Article Snippet: .. The qPCR amplification mixtures contained 3 μL diluted template cDNA, 5 μL 2x miRCURY SYBR Green master mix, 0.5 μL ROX reference dye, 0.5 μL RNAse free water and 1 μL miRCURY LNA miRNA PCR Assay (Qiagen). ..

    Amplification:

    Article Title: Hemoglobinuria associated with Candidatus Mycoplasma Haemobos in a primiparous Holstein cow in Turkey: a case report
    Article Snippet: .. PCR amplification was performed in a 25 μL containing DNase- and RNase-free water (Qiagen ® , Germany), 10× PCR buffer (Thermo ScientificTM, Lithuania), MgCl2 (25 mM; Thermo ScientificTM, Lithuania), 200 μM of each deoxynucleotide triphosphate (dNTP; Thermo ScientificTM, Lithuania), 1.25 U Taq DNA polymerase (Thermo ScientificTM, Lithuania), 1 μL of each primer (10 pmol/μL), and 3 μL of template DNA. ..

    Article Title: Night shift work and epigenetic modifications: cardiovascular-related miRNA expression within a European cohort of night shift workers.
    Article Snippet: .. The qPCR amplification mixtures contained 3 μL diluted template cDNA, 5 μL 2x miRCURY SYBR Green master mix, 0.5 μL ROX reference dye, 0.5 μL RNAse free water and 1 μL miRCURY LNA miRNA PCR Assay (Qiagen). ..



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    Analytical specificity of the quadruplex qPCR assay. (A) Analytical specificity for Mhp detected through the FAM channel (red). (B) Analytical specificity for Mhr detected through the ROX channel (black). (C) Analytical specificity for APP detected through the VIC channel (blue). (D) Analytical specificity for Gps detected through the Cy5 channel (green). In (A–D): 1 = p-Mhp recombinant plasmid; 2 = p-Mhr recombinant plasmid; 3 = p-APP recombinant plasmid; 4 = p-Gps recombinant plasmid; 5 = Mhp (strain J); 6 = Mhr (positive clinical sample); 7 = APP (trivalent vaccine containing serotype 1 strain JL9901, serotype 2 strain XT9904, and serotype 7 strain GZ9903); 8 = Gps (quadrivalent vaccine containing serotype 4 strain SD02, serotype 5 strain HN02, serotype 12 strain GZ01, and serotype 13 strain JX03); 9–12 = Positive clinical samples of Mhp, Mhr, APP, and Gps, respectively; 13 = CSFV; 14 = PCV2; 15 = PRRSV; 16 = PRV; 17 = SIV; 18 = E. coli ; 19 = Group C Streptococcus and S. suis serotype 2; 20 = S. choleraesuis ; 21 = E. rhusiopathiae ; 22 = P. multocida ; 23 <t>=</t> <t>Nuclease-free</t> distilled water. qPCR = Quantitative polymerase chain reaction; Mhp = Mycoplasma hyopneumoniae ; Mhr = Mycoplasma hyorhinis ; APP = Actinobacillus pleuropneumoniae ; Gps = Glaesserella parasuis ; FAM = 6-Carboxyfluorescein; ROX = Carboxy-X-rhodamine; VIC = VIC fluorescent dye; Cy5 = Cyanine 5; CSFV = Classical swine fever virus; PCV2 = Porcine circovirus type 2; PRRSV = Porcine reproductive and respiratory syndrome virus; PRV = Pseudorabies virus; SIV = Swine influenza virus.
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    Analytical specificity of the quadruplex qPCR assay. (A) Analytical specificity for Mhp detected through the FAM channel (red). (B) Analytical specificity for Mhr detected through the ROX channel (black). (C) Analytical specificity for APP detected through the VIC channel (blue). (D) Analytical specificity for Gps detected through the Cy5 channel (green). In (A–D): 1 = p-Mhp recombinant plasmid; 2 = p-Mhr recombinant plasmid; 3 = p-APP recombinant plasmid; 4 = p-Gps recombinant plasmid; 5 = Mhp (strain J); 6 = Mhr (positive clinical sample); 7 = APP (trivalent vaccine containing serotype 1 strain JL9901, serotype 2 strain XT9904, and serotype 7 strain GZ9903); 8 = Gps (quadrivalent vaccine containing serotype 4 strain SD02, serotype 5 strain HN02, serotype 12 strain GZ01, and serotype 13 strain JX03); 9–12 = Positive clinical samples of Mhp, Mhr, APP, and Gps, respectively; 13 = CSFV; 14 = PCV2; 15 = PRRSV; 16 = PRV; 17 = SIV; 18 = E. coli ; 19 = Group C Streptococcus and S. suis serotype 2; 20 = S. choleraesuis ; 21 = E. rhusiopathiae ; 22 = P. multocida ; 23 <t>=</t> <t>Nuclease-free</t> distilled water. qPCR = Quantitative polymerase chain reaction; Mhp = Mycoplasma hyopneumoniae ; Mhr = Mycoplasma hyorhinis ; APP = Actinobacillus pleuropneumoniae ; Gps = Glaesserella parasuis ; FAM = 6-Carboxyfluorescein; ROX = Carboxy-X-rhodamine; VIC = VIC fluorescent dye; Cy5 = Cyanine 5; CSFV = Classical swine fever virus; PCV2 = Porcine circovirus type 2; PRRSV = Porcine reproductive and respiratory syndrome virus; PRV = Pseudorabies virus; SIV = Swine influenza virus.
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    Image Search Results


    Analytical specificity of the quadruplex qPCR assay. (A) Analytical specificity for Mhp detected through the FAM channel (red). (B) Analytical specificity for Mhr detected through the ROX channel (black). (C) Analytical specificity for APP detected through the VIC channel (blue). (D) Analytical specificity for Gps detected through the Cy5 channel (green). In (A–D): 1 = p-Mhp recombinant plasmid; 2 = p-Mhr recombinant plasmid; 3 = p-APP recombinant plasmid; 4 = p-Gps recombinant plasmid; 5 = Mhp (strain J); 6 = Mhr (positive clinical sample); 7 = APP (trivalent vaccine containing serotype 1 strain JL9901, serotype 2 strain XT9904, and serotype 7 strain GZ9903); 8 = Gps (quadrivalent vaccine containing serotype 4 strain SD02, serotype 5 strain HN02, serotype 12 strain GZ01, and serotype 13 strain JX03); 9–12 = Positive clinical samples of Mhp, Mhr, APP, and Gps, respectively; 13 = CSFV; 14 = PCV2; 15 = PRRSV; 16 = PRV; 17 = SIV; 18 = E. coli ; 19 = Group C Streptococcus and S. suis serotype 2; 20 = S. choleraesuis ; 21 = E. rhusiopathiae ; 22 = P. multocida ; 23 = Nuclease-free distilled water. qPCR = Quantitative polymerase chain reaction; Mhp = Mycoplasma hyopneumoniae ; Mhr = Mycoplasma hyorhinis ; APP = Actinobacillus pleuropneumoniae ; Gps = Glaesserella parasuis ; FAM = 6-Carboxyfluorescein; ROX = Carboxy-X-rhodamine; VIC = VIC fluorescent dye; Cy5 = Cyanine 5; CSFV = Classical swine fever virus; PCV2 = Porcine circovirus type 2; PRRSV = Porcine reproductive and respiratory syndrome virus; PRV = Pseudorabies virus; SIV = Swine influenza virus.

    Journal: Veterinary World

    Article Title: Development and validation of a quadruplex real-time quantitative polymerase chain reaction assay for the simultaneous detection of Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Actinobacillus pleuropneumoniae, and Glaesserella parasuis

    doi: 10.14202/vetworld.2026.3944-3960

    Figure Lengend Snippet: Analytical specificity of the quadruplex qPCR assay. (A) Analytical specificity for Mhp detected through the FAM channel (red). (B) Analytical specificity for Mhr detected through the ROX channel (black). (C) Analytical specificity for APP detected through the VIC channel (blue). (D) Analytical specificity for Gps detected through the Cy5 channel (green). In (A–D): 1 = p-Mhp recombinant plasmid; 2 = p-Mhr recombinant plasmid; 3 = p-APP recombinant plasmid; 4 = p-Gps recombinant plasmid; 5 = Mhp (strain J); 6 = Mhr (positive clinical sample); 7 = APP (trivalent vaccine containing serotype 1 strain JL9901, serotype 2 strain XT9904, and serotype 7 strain GZ9903); 8 = Gps (quadrivalent vaccine containing serotype 4 strain SD02, serotype 5 strain HN02, serotype 12 strain GZ01, and serotype 13 strain JX03); 9–12 = Positive clinical samples of Mhp, Mhr, APP, and Gps, respectively; 13 = CSFV; 14 = PCV2; 15 = PRRSV; 16 = PRV; 17 = SIV; 18 = E. coli ; 19 = Group C Streptococcus and S. suis serotype 2; 20 = S. choleraesuis ; 21 = E. rhusiopathiae ; 22 = P. multocida ; 23 = Nuclease-free distilled water. qPCR = Quantitative polymerase chain reaction; Mhp = Mycoplasma hyopneumoniae ; Mhr = Mycoplasma hyorhinis ; APP = Actinobacillus pleuropneumoniae ; Gps = Glaesserella parasuis ; FAM = 6-Carboxyfluorescein; ROX = Carboxy-X-rhodamine; VIC = VIC fluorescent dye; Cy5 = Cyanine 5; CSFV = Classical swine fever virus; PCV2 = Porcine circovirus type 2; PRRSV = Porcine reproductive and respiratory syndrome virus; PRV = Pseudorabies virus; SIV = Swine influenza virus.

    Article Snippet: Each 20 μL reaction contained 10 μL of 2× Premix Ex Taq (Probe qPCR) (Code No. RR390A; TaKaRa, Dalian, China), 0.1-0.6 μL each of primers and probes (20 μM), 2.0 μL of the mixed standard plasmids (1 × 108 copies/μL), and nuclease-free water (Code No. 9012; TaKaRa, Dalian, China) to a final volume of 20 μL.

    Techniques: Recombinant, Plasmid Preparation, Real-time Polymerase Chain Reaction, Virus